PXD026814-1

PXD026814 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analysis reveals reprogrammed NAD+ metabolism in ZIKA virus-induced microcephaly with therapeutic implications
Description	ZIKV-infection during pregnancy can cause microcephaly in newborns, yet the underlying mechanisms remain largely unexplored. Here, we reveal extensive and large-scale metabolic reprogramming events in ZIKV-infected mouse brains. We perform a multi-omics study comprising transcriptomics, proteomics, phosphoproteomics, and metabolomics approaches on the brains from ZIKV-infected mice. Our proteomics and metabolomics analyses uncover dramatic alteration of nicotinamide adenine dinucleotide (NAD+)-related metabolic pathways, including oxidative phosphorylation, TCA cycle, and tryptophan metabolism. Phosphoproteomics analysis indicates that MAPK and cGMP-PKG signalling may be associated with ZIKV-induced microcephaly. Importantly, we demonstrate the utility of our rich multi-omics datasets with follow-up in vivo experiments which confirm that boosting NAD+ by NAD+ or NR supplementation alleviates cell death and increases cortex thickness in ZIKV-infected mouse brains. NR supplementation increases the brain and body weight as well as improves the survival in ZIKV-infected mice. Our study provides a comprehensive resource of biological data to support future investigations of ZIKV-induced microcephaly, and demonstrates that metabolic alterations can be potentially exploited for developing therapeutic strategies.
HostingRepository	iProX
AnnounceDate	2021-06-18
AnnouncementXML	Submission_2021-07-25_23:16:50.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yushen Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-20 19:41:35	ID requested
⏵ 1	2021-07-25 23:16:50	announced
2	2021-12-07 19:11:26	announced	2021-12-08: Update publication information.

Dataset with its publication pending

submitter keyword: ZIKA virus, Microcephaly, Transcriptomics, Proteomics,Phosphoproteomics,Metabolomics, MAD+ metabolism

Zeping Hu
contact affiliation	Tsinghua University
contact email	zeping_hu@tsinghua.edu.cn
lab head
Yushen Wang
contact affiliation	Tsinghua University
contact email	wangys17@mails.tsinghua.edu.cn
dataset submitter

iProX dataset URI