PXD026772-1

PXD026772 is an original dataset announced via ProteomeXchange.

Title	Investigation of cell wall proteins of Camellia sinensis leaves by combining cell wall proteomics and N-glycoproteomics
Description	Cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification of C. sinensis leaves. However, there has been a lack in CWP identification and characterization up to now. This study is aimed to characterize cell wall proteome of C. sinensis leaves and to develop more CWPs related to stress response. Cell wall proteomics and N-glycoproteomics was employed to investigate CWPs. CWPs were extracted by sequential salt buffers, while N-glycoproteins were enriched by hydrophilic interaction chromatography method, and subsequently all proteins were subjected to UPLC-MS/MS analysis. A total of 501 CWPs and 195 CWPs were identified respectively by cell wall proteomics and N-glycoproteomics profiling with 118 CWPs in common. Among identified CWPs, proteins acting on cell wall polysaccharides, proteases and oxidoreductases represent the top three functional groups. This was the first large-scale investigation of CWPs in C. sinensis.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_04:39:19.703.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanli Liu
SpeciesList	scientific name: Camellia sinensis; NCBI TaxID: 4442;
ModificationList	N-glycosylated residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-18 01:28:24	ID requested
⏵ 1	2022-02-17 04:39:20	announced

Liu Y, Ma L, Cao D, Gong Z, Fan J, Hu H, Jin X, Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics. BMC Plant Biol, 21(1):384(2021) [pubmed]

submitter keyword: Camellia sinensis

leaves

cell wall proteins;

Yanli Liu
contact affiliation	Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences
contact email	zkslyl@16.com
lab head
Yanli Liu
contact affiliation	Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences
contact email	zkslyl@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026772
     1. Label: PRIDE project
     2. Name: Investigation of cell wall proteins of Camellia sinensis leaves by combining cell wall proteomics and N-glycoproteomics