PXD026763-1

PXD026763 is an original dataset announced via ProteomeXchange.

Title	Human MDA-MB-231 breast cancer cells overexpressing miR-183-5p and its isomiRs
Description	Overexpression of miR-183-5p|+2, but not of the other two isomiRs |0 and |+1, was observed to reduce cell cycle and cell proliferation in different triple-negative breast cancer cell lines. Therefore, we hypothesized that the |+2 isoform has targets distinct from the other two isoforms. To test this hypothesis, we overexpressed separately the three different isoforms or negative controls (siAllstar or mimic-Cltr) and performed Mass Spectrometry to identify differentially regulated proteins. Interestingly, a gene set enrichment analysis of the changes in protein expression revealed significant downregulation of transcriptional targets of E2F specifically in cells transfected with the |+2 isoform prompting us to validate the predicted isomiR specific target E2F1. Subsequently, we could show that direct targeting of E2F1 by miR-183-5p|+2 is responsible for the impact of the isomiR on cell cycle and proliferation.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-26_16:05:54.425.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cindy Körner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-17 21:36:13	ID requested
⏵ 1	2022-05-26 16:05:54	announced

Dataset with its publication pending

submitter keyword: Human, breast cancer, MDA-MB-231, miRNA, miR-183-5p

Stefan Wiemann
contact affiliation	Molecular Genome Analysis, German Cancer Research Centre, Heidelberg, Germany
contact email	s.wiemann@dkfz.de
lab head
Cindy Körner
contact affiliation	German Cancer Research Center, Heidelberg Molecular Genome Analysis (B050)
contact email	c.koerner@dkfz-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD026763

PRIDE project URI

• PRIDE
  1. PXD026763
     1. Label: PRIDE project
     2. Name: Human MDA-MB-231 breast cancer cells overexpressing miR-183-5p and its isomiRs