PXD026737-2
PXD026737 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Legionella pneumophila life stage-specific proteins regulated via ClpP-dependent |
| Description | To investigate the role of ClpP-mediated proteolysis in the regulation of life stage specific proteins of Legionella pneumophila, the bacteria in the RP/TP of WT and ΔclpP were collected and analyzed by mass spectrometry. To screen the substrates of ClpP during the whole life cycle, bacterial whole-cell lysates from clpPwt and clpPtrap in the TP were prepared and His-tagged proteins were purified with Ni-NTA affinity column (GE Healthcare) following the manufacturer’s instructions. Substrates captured inside the proteolytic barrel were co-purified along with the His-tagged ClpP complex and identified by mass spectrometry to identify substrates of ClpP in the WT background. Each sample was analyzed in biological triplicates to allow for statistical tests and to improve consistency. Protein identification and quality criteria were very strict throughout the study. |
| HostingRepository | iProX |
| AnnounceDate | 2021-06-16 |
| AnnouncementXML | Submission_2023-02-02_19:05:26.959.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhenhuang Ge |
| SpeciesList | scientific name: Legionella pneumophila subsp. pneumophila Lp02; NCBI TaxID: 1080311; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-06-16 00:16:18 | ID requested | |
| 1 | 2021-06-16 00:16:44 | announced | |
| ⏵ 2 | 2023-02-02 19:05:28 | announced | 2023-02-03: Update publication information. |
Publication List
| Ge Z, Yuan P, Chen L, Chen J, Shen D, She Z, Lu Y, New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila. Mol Cell Proteomics, 21(5):100233(2022) [pubmed] |
Keyword List
| submitter keyword: Legionella pneumophila, biphasic life cycle, regulatory protein, effector protein, substrate protein |
Contact List
| Yongjun Lu | |
|---|---|
| contact affiliation | Sun Yat-sen University |
| contact email | luyj@mail.sysu.edu.cn |
| lab head | |
| Zhenhuang Ge | |
| contact affiliation | Sun Yat-sen University |
| contact email | 938370861@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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