PXD026725-1

PXD026725 is an original dataset announced via ProteomeXchange.

Title	RNA-bound PGC-1α controls gene expression in liquid-like nuclear condensates
Description	The peroxisome-proliferator-activated receptor-γ coactivator-1α (PGC-1α) integrates environmental cues by controlling complex transcriptional networks in various metabolically active tissues. However, it is unclear how a transcriptional coregulator coordinates dynamic biological programs in response to multifaceted stimuli such as endurance training or fasting. Here, we discovered a central function of the poorly understood C-terminal domain (CTD) of PGC-1α to bind RNAs and assemble multi-protein complexes. Surprisingly, in addition to controlling the coupling of transcription and processing of target genes, RNA binding is indispensable for the recruitment of PGC-1α to chromatin into liquid-like nuclear condensates, which compartmentalize and regulate active transcription. These results demonstrate a hitherto unsuspected molecular mechanism by which complexity in the regulation of large transcriptional networks by PGC-1α is achieved. These findings are not only essential for the basic understanding of transcriptional coregulator-driven control of biological programs, but will also help to devise new strategies to modulate these processes in pathological contexts in which PGC-1α function is dysregulated, such as type 2 diabetes, cardiovascular diseases or skeletal muscle wasting.
HostingRepository	PRIDE
AnnounceDate	2021-08-19
AnnouncementXML	Submission_2021-08-19_05:30:17.036.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026725
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Danilo Ritz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-15 14:37:07	ID requested
⏵ 1	2021-08-19 05:30:17	announced

Dataset with its publication pending

submitter keyword: Muscle cells, APMS, LCMS/MS

Alexander Schmidt
contact affiliation	Dr. Alexander Schmidt Proteomics Core Facility Biozentrum, University of Basel Klingelbergstrasse 50/70 CH-4056 Basel / Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Danilo Ritz
contact affiliation	Proteomics Core Facility Biozentrum, University of Basel Klingelbergstrasse 50/70 CH-4056 Basel / Switzerland
contact email	danilo.ritz@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026725
     1. Label: PRIDE project
     2. Name: RNA-bound PGC-1α controls gene expression in liquid-like nuclear condensates