PXD026724-1

PXD026724 is an original dataset announced via ProteomeXchange.

Title	Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response
Description	Caveolae-associated protein 3 (cavin3), a putative tumor suppressor protein, is inactivated in most cancers. The molecular basis for its actions remains poorly understood. We characterized the effects on the proteome of cavin3 loss by utilizing genome-edited cells together with label-free quantitative proteomics. These studies revealed a prominent role for cavin3 in DNA repair pathways with downregulation of BRCA1 and the BRCA1 A-complex components. Using cellular and cell-free expression assays, we show a direct interaction between BRCA1 and cavin3. Association of BRCA1 and cavin3 occurs when cavin3 is released from disassembling caveolae by multiple stressors such as UV and mechanical stress. Cavin3-deficient cells were sensitised to PARP inhibition and showed a loss of components of the BRCA1 A-complex associated with UV DNA damage foci. Overexpression and RNAi-depletion strategies in several cancer cell lines revealed that cavin3 sensitized a variety of cancer cells to UV-induced apoptosis. We conclude that cavin3 functions together with BRCA1 in multiple pathways that contribute to tumorigenesis.
HostingRepository	PRIDE
AnnounceDate	2021-06-15
AnnouncementXML	Submission_2021-06-15_15:56:30.071.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Stroud
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-15 14:33:25	ID requested
⏵ 1	2021-06-15 15:56:30	announced

Dataset with its publication pending

submitter keyword: BRCA1, breast cancer, caveolae, cavin proteins

David Stroud
contact affiliation	Department of Biochemistry & Pharmacology, University of Melbourne
contact email	david.stroud@unimelb.edu.au
lab head
David Stroud
contact affiliation	The University of Melbourne
contact email	david.stroud@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026724
     1. Label: PRIDE project
     2. Name: Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response