PXD026720-1

PXD026720 is an original dataset announced via ProteomeXchange.

Title	Identification of CDK9 targets using DRB and quantitative phosphoproteomics
Description	CDK9 is a critical kinase required for the productive transcription of protein-coding genes by RNA polymerase II (pol II) in higher eukaryotes. Phosphorylation of targets including the elongation factor SPT5 and the carboxyl-terminal domain (CTD) of RNA pol II allow the polymerase to pass an early elongation checkpoint (EEC), which is encountered soon after initiation. In addition to halting RNA polymerase II at the EEC, CDK9 inhibition also causes premature termination of transcription across the last exon, loss of polyadenylation factors from chromatin, and loss of polyadenylation of nascent transcripts. Inhibition of the phosphatase PP2A abrogates the premature termination and loss of polyadenylation caused by CDK9 inhibition, suggesting that CDK9 and PP2A, working together, regulate the coupling of elongation and transcription termination to RNA maturation. Our phosphoproteomic analyses, using either DRB or an analog-sensitive CDK9 cell line confirm the splicing factor SF3B1 as an additional key target of this kinase. CDK9 inhibition causes loss of interaction of splicing and export factors with SF3B1, suggesting that CDK9 also helps to co-ordinates coupling of splicing and export to transcription.
HostingRepository	PRIDE
AnnounceDate	2022-08-22
AnnouncementXML	Submission_2022-08-22_05:46:13.439.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Tellier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-15 08:15:28	ID requested
⏵ 1	2022-08-22 05:46:14	announced

Tellier M, Zaborowska J, Neve J, Nojima T, Hester S, Fournier M, Furger A, Murphy S, CDK9 and PP2A regulate RNA polymerase II transcription termination and coupled RNA maturation. EMBO Rep, 23(10):e54520(2022) [pubmed]

submitter keyword: SILAC, phosphoproteomics, CDK9, DRB, HeLa

Shona Murphy
contact affiliation	Sir William Dunn School of Pathology, University of Oxford,Oxford, UK
contact email	shona.murphy@path.ox.ac.uk
lab head
Michael Tellier
contact affiliation	Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
contact email	michael.tellier@path.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD026720

PRIDE project URI

• PRIDE
  1. PXD026720
     1. Label: PRIDE project
     2. Name: Identification of CDK9 targets using DRB and quantitative phosphoproteomics