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PXD026720 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of CDK9 targets using DRB and quantitative phosphoproteomics
DescriptionCDK9 is a critical kinase required for the productive transcription of protein-coding genes by RNA polymerase II (pol II) in higher eukaryotes. Phosphorylation of targets including the elongation factor SPT5 and the carboxyl-terminal domain (CTD) of RNA pol II allow the polymerase to pass an early elongation checkpoint (EEC), which is encountered soon after initiation. In addition to halting RNA polymerase II at the EEC, CDK9 inhibition also causes premature termination of transcription across the last exon, loss of polyadenylation factors from chromatin, and loss of polyadenylation of nascent transcripts. Inhibition of the phosphatase PP2A abrogates the premature termination and loss of polyadenylation caused by CDK9 inhibition, suggesting that CDK9 and PP2A, working together, regulate the coupling of elongation and transcription termination to RNA maturation. Our phosphoproteomic analyses, using either DRB or an analog-sensitive CDK9 cell line confirm the splicing factor SF3B1 as an additional key target of this kinase. CDK9 inhibition causes loss of interaction of splicing and export factors with SF3B1, suggesting that CDK9 also helps to co-ordinates coupling of splicing and export to transcription.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Tellier
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Velos; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-15 08:15:28ID requested
12022-08-22 05:46:14announced
Publication List
Tellier M, Zaborowska J, Neve J, Nojima T, Hester S, Fournier M, Furger A, Murphy S, CDK9 and PP2A regulate RNA polymerase II transcription termination and coupled RNA maturation. EMBO Rep, 23(10):e54520(2022) [pubmed]
Keyword List
submitter keyword: SILAC, phosphoproteomics, CDK9, DRB, HeLa
Contact List
Shona Murphy
contact affiliationSir William Dunn School of Pathology, University of Oxford,Oxford, UK
contact emailshona.murphy@path.ox.ac.uk
lab head
Michael Tellier
contact affiliationSir William Dunn School of Pathology, University of Oxford, Oxford, UK.
contact emailmichael.tellier@path.ox.ac.uk
dataset submitter
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Dataset FTP location
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