PXD026689-1

PXD026689 is an original dataset announced via ProteomeXchange.

Title	Development and characterization of vorinostat resistant cutaneous T cell lymphoma cells
Description	Vorinostat is the very first approved inhibitor of histone deacetylase, widely used in many countries for the treatment of primary/ persistent cutaneous T cell lymphoma (CTCL). However, problems associated with intrinsic and acquired resistance to vorinostat present a significant challenge to therapy. Here, we present our in vitro findings on the development and characterization of acquired resistance to vorinostat in a CTCL line HuT78 (HuT78VR). Employing an integrated transcriptomics and proteomics approach, we identified molecular signatures of vorinostat resistance in HuT78VR. We noted defective apoptosis, enhanced drug-efflux ability, upregulation of metastasis-related genes and mitogen-activated protein kinase signaling, increased cellular invasiveness, and downregulation of caspase 3 and phosphorylated forms of STAT3 in HuT78VR cells. HuT78VR cells gained cross-resistance to commonly used inhibitors of histone deacetylase and topoisomerase. Intriguingly, HuT78VR cells remained sensitive to inhibitors of STAT3, Janus-associated kinase, and extracellular receptor kinase, suggesting their pertinence in the management of vorinostat resistant CTCL.
HostingRepository	PRIDE
AnnounceDate	2026-09-08
AnnouncementXML	Submission_2026-09-07_19:25:00.228.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yee Jiun Kok
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-14 05:35:52	ID requested
⏵ 1	2026-09-07 19:25:00	announced

Dataset with its publication pending

submitter keyword: ERK,Vorinostat resistance, SILAC proteomics, RNAseq analysis

Xuezhi Bi
contact affiliation	Bioprocessing Technology Institute
contact email	bi_xuezhi@bti.a-star.edu.sg
lab head
Yee Jiun Kok
contact affiliation	Bioprocessing Technology Institute, A*STAR
contact email	kok_yee_jiun@a-star.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD026689

PRIDE project URI

• PRIDE
  1. PXD026689
     1. Label: PRIDE project
     2. Name: Development and characterization of vorinostat resistant cutaneous T cell lymphoma cells