PXD026683 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Impact of pregnancy on composition of human high density lipoproteins (SEC fractions) |
Description | Pregnancy results in significant physiological changes which can impact the health and development of the fetus and mother. Pregnancy-induced changes in plasma lipoproteins are well-documented with modest to no impact observed on the generic measure of high-density lipoprotein (HDL) cholesterol. However, no studies have taken a deeper look at the impact of pregnancy on the concentration and composition of HDL subspecies. In this prospective study, we collected plasma from 24 non-pregnant and 19 pregnant women in their second trimester. Using nuclear magnetic resonance (NMR) we quantified 11 different lipoprotein size subspecies from plasma including 3 in the HDL class. We observed a profound increase in the number of larger HDL particles in pregnant women. These findings were confirmed by tracking phospholipid across lipoproteins using high-resolution gel-filtration chromatography. Using liquid chromatography-mass spectrometry (LC-MS) we identified 87 lipid-associated proteins across size speciated fractions. We report drastic shifts in multiple protein clusters across different HDL sized fractions in pregnant females compared to their non-pregnant controls. In summary, we have shown that pregnancy results in major alterations in HDL subspecies concentrations and compositions that would have otherwise been missed using traditional lipid measurements. These findings significantly elevate our understanding of how changes in lipoprotein metabolism due to pregnancy could impact the health of both the fetus and the mother. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_04:46:36.646.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | John Melchior |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | 6550 iFunnel Q-TOF LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-14 05:35:09 | ID requested | |
⏵ 1 | 2022-02-17 04:46:37 | announced | |
Publication List
Melchior JT, Swertfeger DK, Morris J, Street SE, Warshak CR, Welge JA, Remaley AT, Catov JM, Davidson WS, Woollett LA, Pregnancy is accompanied by larger high density lipoprotein particles and compositionally distinct subspecies. J Lipid Res, 62():100107(2021) [pubmed] |
Keyword List
submitter keyword: Plasma, Pregnancy, LC-MS/MS, proteomics, HDL |
Contact List
Laura Woollett |
contact affiliation | University of Cincinnati Department of Pathology and Laboratory Medicine |
contact email | woollela@ucmail.uc.edu |
lab head | |
John Melchior |
contact affiliation | Pacific Northwest National Laboratories University of Cincinnati |
contact email | john.melchior@pnnl.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026683
- Label: PRIDE project
- Name: Impact of pregnancy on composition of human high density lipoproteins (SEC fractions)