PXD026674-1

PXD026674 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation
Description	The Microrchidia (MORC) family of ATPases are required for transposable element (TE) silencing and heterochromatin condensation in plants and animals, and C. elegans MORC-1 has been shown to topologically entrap and condense DNA. In Arabidopsis thaliana, mutation of MORCs has been shown to reactivate silent methylated genes and transposons and to decondense heterochromatic chromocenters, despite only minor changes in the maintenance of DNA methylation. Here we provide the first evidence localizing Arabidopsis MORC proteins to specific regions of chromatin and find that MORC4 and MORC7 are closely co-localized with sites of RNA directed DNA methylation (RdDM). We further show that MORC7, when tethered to DNA by an artificial zinc finger, can facilitate the establishment of RdDM. Finally, we show that MORCs are required for the efficient RdDM mediated establishment of DNA methylation and silencing of a newly integrated FWA transgene, even though morc mutations have no effect on the maintenance of preexisting methylation at the endogenous FWA gene. We propose that MORCs function as a molecular tether in RdDM complexes to reinforce RdDM activity for methylation establishment. These findings have implications for MORC protein function in a variety of other eukaryotic organisms.
HostingRepository	PRIDE
AnnounceDate	2021-07-02
AnnouncementXML	Submission_2021-07-02_10:40:43.201.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026674
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhong Zhenhui
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-14 05:34:10	ID requested
⏵ 1	2021-07-02 10:40:43	announced

Dataset with its publication pending

submitter keyword: Arabidopsis MORC7

Yan Xue
contact affiliation	UCLA
contact email	y.xue16@gmail.com
lab head
Zhong Zhenhui
contact affiliation	UCLA
contact email	zhenhuizhong@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026674
     1. Label: PRIDE project
     2. Name: Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation