PXD026646 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LFQ-proteomic on bbs1 zebrafish photoreceptor outer segments |
Description | Bardet-Biedl syndrome (BBS) is a ciliopathy characterized by retinal dystrophy, intellectual disability, polydactyly, obesity and renal anomalies. In photoreceptors (PR), light sensation occurs in outer segments (OSs), which are specialized primary cilia. BBS1, the major BBS gene, is part of a protein complex called “BBSome”, which is involved in intracellular protein transport. However, the precise function of BBS1/BBSome in controlling trafficking of ciliary proteins in PRs remains unclear. To investigate the role of the BBSome in photoreceptors, we conducted a label free quantitative proteomic investigation on the protein contend of isolated adult mutant and control outer segments. We revealed that a Bbs1 KO results in the loss of the entire BBSome from OSs. Besides the loss of the BBSome we found an overall accumulation of non-outer segment proteins in the OS. A majority of these proteins were membrane-associated, supporting the role of BBS1/the BBSome in controlling ciliary transport of membrane-associated proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-10 |
AnnouncementXML | Submission_2022-02-10_14:28:28.609.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Markus Masek |
SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-11 06:28:56 | ID requested | |
⏵ 1 | 2022-02-10 14:28:28 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Zebrafish, Photoreceptor, Outer segment, LFQ-P, BBS |
Contact List
Ruxandra Bachmann- Gagescu |
contact affiliation | Institute of Medical Genetics, Bachmann-Lab, University of Zurich |
contact email | ruxandra.bachmann@mls.uzh.ch |
lab head | |
Markus Masek |
contact affiliation | University of Zurich |
contact email | markus.masek@uzh.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026646 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026646
- Label: PRIDE project
- Name: LFQ-proteomic on bbs1 zebrafish photoreceptor outer segments