PXD026641 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hypoxia induces a transcriptional primitive streak signature in ES cells and enhances lineage patterning in gastruloids |
Description | The cellular microenvironment shapes stem cell identity and differentiation capacity. Mammalian early embryos are exposed to hypoxia in vivo and benefit from hypoxic culture in vitro. Yet, how different components of the hypoxia response impact stem cell transcriptional networks and lineage choices remains unclear. Here we investigated the effect of acute and prolonged hypoxia on stem cell states and differentiation efficiencies of embryonic and extraembryonic cells. We show that prolonged hypoxia enhances differentiation of embryonic stem (ES) cells towards the mesendoderm lineage by transcriptionally priming cells with a primitive streak signature including Wnt3 and T expression. Exposure to hypoxia in ES culture or during formation of gastrulation-mimicking organoids (gastruloids) moderates T expression and enhances structural complexity. Hypoxic gastruloids generated without exogenous Wnt induction can spontaneously elongate and self-organize. Direct gene regulation by Hif1a, combined with DNA demethylation and metabolic rewiring modulate the transcriptional response and phenotypic outcome. Our findings highlight the influence of the microenvironment on stem cell function and provide a rationale supportive of applying physiological conditions in synthetic embryo models. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-05 |
AnnouncementXML | Submission_2022-09-05_02:48:14.992.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Meierhofer |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-11 02:56:20 | ID requested | |
⏵ 1 | 2022-09-05 02:48:15 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: early embryos, hypoxia, embryonic stem |
Contact List
Aydan Bulut-Karslioglu |
contact affiliation | Sofja Kovaleskaja Research Group, Max Planck Institute for Molecular Genetics, Berlin, Germany |
contact email | Karsliog@molgen.mpg.de |
lab head | |
David Meierhofer |
contact affiliation | Mass Spectrometry Facility MPIMG |
contact email | meierhof@molgen.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026641
- Label: PRIDE project
- Name: Hypoxia induces a transcriptional primitive streak signature in ES cells and enhances lineage patterning in gastruloids