PXD026631-1

PXD026631 is an original dataset announced via ProteomeXchange.

Title	Repetition of comparison of sample preservation methods for field collection of animal-microbe symbioses for metaproteomic analyses
Description	When studying gene expression in microbe-animals symbioses collected in the field it is essential to quickly and efficiently preserve in situ symbiont and host protein abundance patterns. One of the most commonly used sample preservation methods for samples targeted for proteomic analyses is flash freezing, however, liquid nitrogen or dry ice needed for flash freezing are often not available at remote field sites. We replicated our experiment from PXD014591 to test if RNAlater allows preserving proteins in animal-microbe symbioses as efficiently as flash freezing and without introducing issues with downstream processing. We used the marine gutless oligochaete Olavius algarvensis as a model for testing. Olavius algarvensis lives in shallow water sediments off the coast of Elba, Italy. It has no digestive and excretory system and harbors five bacterial symbionts that fulfill its nutritional and waste recycling needs (Kleiner et al., 2012, PNAS 109(19):1173-82). We compared six RNAlater preserved and eight flash frozen samples in terms of the number of identified proteins, abundances of individual proteins and potential biases against specific protein or taxonomic groups. Six worms were incubated in RNAlater for 24 hours. After incubation, RNAlater was removed and samples were stored at -80°C. Eight worms were directly flash frozen in liquid nitrogen and stored at -80 °C immediately after preservation.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_04:49:45.625.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlene Jensen
SpeciesList	scientific name: Olavius algarvensis spirochete endosymbiont; NCBI TaxID: 260710; scientific name: Olavius algarvensis Gamma 1 endosymbiont; NCBI TaxID: 260705; scientific name: Olavius algarvensis sulfate-reducing endosymbiont; NCBI TaxID: 146479; scientific name: Olavius algarvensis; NCBI TaxID: 188229; scientific name: Olavius algarvensis Delta 1 endosymbiont; NCBI TaxID: 334747; scientific name: Olavius algarvensis Delta 4 endosymbiont; NCBI TaxID: 260707;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-10 22:17:57	ID requested
⏵ 1	2021-11-03 04:49:46	announced
2	2023-09-28 03:42:31	announced	2023-09-28: Updated project metadata.

Dataset with its publication pending

submitter keyword: 1D-LC, metaproteomics, metaproteome, chemosynthetic symbiosis, gutless oligochaetes, Olavius algarvensis, proteobacteria, thiotrophic endosymbionts, RNAlater, sample preservation, field samples, microbiom

Manuel Kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
Marlene Jensen
contact affiliation	North Carolina State University
contact email	mjensen2@ncsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026631
     1. Label: PRIDE project
     2. Name: Repetition of comparison of sample preservation methods for field collection of animal-microbe symbioses for metaproteomic analyses