PXD026619-1

PXD026619 is an original dataset announced via ProteomeXchange.

Title	Dynamic character displacement among a pair of bacterial phyllosphere commensals in situ
Description	Differences between species promote stable coexistence in a resource-limited environment. These differences can result from interspecies competition leading to character shifts, a process referred to as character displacement. While character displacement is often interpreted as a consequence of genetically fixed trait differences between species, it can also be mediated by phenotypic plasticity in response to the presence of another species. Here, we test whether phenotypic plasticity leads to a shift in proteome allocation during co-occurrence of two bacterial species from the abundant, leaf-colonizing families Sphingomonadaceae and Rhizobiaceae in their natural habitat. Upon mono-colonizing of the phyllosphere, both species exhibit specific and shared protein functions indicating a niche overlap. During co-colonization, quantitative differences in the protein repertoire of both bacterial populations occur as a result of bacterial coexistence in planta. Specifically, the Sphingomonas strain produces enzymes for the metabolization of xylan, while the Rhizobium strain reprograms its metabolism to beta-oxidation of fatty acids fueled via the glyoxylate cycle and adapts its biotin acquisition. We demonstrate the conditional relevance of cross-species facilitation by mutagenesis leading to loss of fitness in competition in planta. Our results show that dynamic character displacement and niche facilitation mediated by phenotypic plasticity can contribute to species coexistence.
HostingRepository	PRIDE
AnnounceDate	2022-05-26
AnnouncementXML	Submission_2022-05-26_09:11:32.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lucas Hemmerle
SpeciesList	scientific name: Sphingomonas sp. Leaf257; NCBI TaxID: 1736309; scientific name: Rhizobium sp. Leaf68; NCBI TaxID: 1736231;
ModificationList	carbamoylated residue; iodoacetamide derivatized amino-terminal residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-10 07:54:25	ID requested
⏵ 1	2022-05-26 09:11:32	announced
2	2023-11-14 08:56:51	announced	2023-11-14: Updated project metadata.

10.1038/S41467-022-30469-3;

submitter keyword: Phyllosphere, Sphingomonas, Rhizobium, Proteomics, Dynamic character displacement

Julia A. Vorholt
contact affiliation	Department of Biology, Institute of Microbiology, ETH Zürich, Switzerland
contact email	jvorholt@ethz.ch
lab head
Lucas Hemmerle
contact affiliation	ETH Zürich
contact email	helucas@ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026619
     1. Label: PRIDE project
     2. Name: Dynamic character displacement among a pair of bacterial phyllosphere commensals in situ