PXD026599-1

PXD026599 is an original dataset announced via ProteomeXchange.

Title	A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism
Description	Members of the Protein Kinase D (PKD) family (PKD1, 2, and 3) integrate hormonal and nutritional inputs to regulate complex cellular metabolism. Despite the fact that a number of functions have been annotated to particular PKDs, their molecular targets are relatively poorly explored. PKD3 promotes insulin sensitivity and suppresses lipogenesis in the liver of animals fed a high-fat diet. However, its substrates are largely unknown. Here we applied proteomic approaches to determine PKD3 targets. We identified over three-hundred putative targets of PKD3. Further, biochemical analysis revealed that PKD3 regulates cAMP-dependent protein kinase A (PKA) activity, a master regulator of the hepatic response to glucagon and fasting. PKA regulates glucose, lipid, and amino acid metabolism in the liver, by targeting key enzymes in the respective processes. Among them the PKA targets phenylalanine hydroxylase (PAH) catalyzes the conversion of phenylalanine to tyrosine Consistently, we showed that PKD3 is activated by glucagon and promotes glucose and tyrosine levels in hepatocytes
HostingRepository	PRIDE
AnnounceDate	2021-06-17
AnnouncementXML	Submission_2021-06-16_22:52:05.966.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angel Loza Valdes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-09 06:15:19	ID requested
⏵ 1	2021-06-16 22:52:06	announced

Dataset with its publication pending

submitter keyword: Primary mouse hepatocytes, PKD3, PKA

Grzegorz Sumara
contact affiliation	nencki institute of experimental biology
contact email	g.sumara@nencki.edu.pl
lab head
Angel Loza Valdes
contact affiliation	PhD Student
contact email	a.loza-valdes@nencki.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026599
     1. Label: PRIDE project
     2. Name: A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism