PXD026570-1

PXD026570 is an original dataset announced via ProteomeXchange.

Title	Extensive Three-Dimensional Intratumor Proteomic Heterogeneity Revealed by Multiregion Sampling in High-Grade Serous Ovarian Tumor Specimens
Description	Enriched tumor epithelium, tumor-associated stroma, and whole tissue were collected by laser microdissection from thin sections across spatially separated levels of ten high-grade serous ovarian carcinomas (HGSOC) and analyzed by mass spectrometry. Unsupervised analyses of protein abundance data revealed independent clustering of enriched stroma and enriched tumor epithelium, with whole tumor tissue clustering driven by overall tumor “purity”. Comparing these data to previously defined prognostic HGSOC molecular subtypes revealed protein expression from tumor epithelium correlated with the differentiated subtype, whereas stromal proteins correlated with the mesenchymal subtype. Protein abundance in tumor epithelium and stroma exhibited decreased correlation in samples collected just hundreds of microns apart. These data reveal substantial tumor microenvironment protein heterogeneity that directly bears on prognostic signatures, biomarker discovery, and cancer pathophysiology, and underscore the need to enrich cellular subpopulations for expression profiling.
HostingRepository	PRIDE
AnnounceDate	2021-07-01
AnnouncementXML	Submission_2021-06-30_23:16:33.934.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brian Hood
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-08 09:04:39	ID requested
⏵ 1	2021-06-30 23:16:34	announced

Dataset with its publication pending

submitter keyword: high grade serous ovarian cancer, proteomics, laser microdissection, tumor microenvironment, heterogeneity

Thomas P. Conrads, PhD
contact affiliation	Director of Women’s Health Research Women’s Service Line Inova Health System Chief Scientific Officer Gynecologic Cancer Center of Excellence John P. Murtha Cancer Center Research Program Uniformed Services University of the Health Sciences
contact email	conrads@whirc.org
lab head
Brian Hood
contact affiliation	Women's Health Integrated Research Center
contact email	hoodb@whirc.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD026570

PRIDE project URI

• PRIDE
  1. PXD026570
     1. Label: PRIDE project
     2. Name: Extensive Three-Dimensional Intratumor Proteomic Heterogeneity Revealed by Multiregion Sampling in High-Grade Serous Ovarian Tumor Specimens