PXD026547 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cells mitochondria and evolved via association of differentially adapted subunits |
Description | In Cleomaceae species, NAD-malic enzyme (NAD-ME) was independently co-opted to participate in C4 photosynthesis. In C4 Cleome species, all NAD-ME genes (NAD-MEα, -ß1 and -ß2) were affected by C4 evolution and are expressed at higher levels than their C3 orthologs in Tarenaya hassleriana. In C3 Cleome, the NAD-ME housekeeping function is performed by two heteromers, NAD-MEα/ß1 and NAD-MEα/ß2, with similar biochemical properties and tissue occurrence. In the C4 species, Gynandropsis gynandra and Cleome angustifolia, this role is performed only by the NAD-MEα/ß2 heteromer. In these C4 species NAD-MEα/ß1 is preferentially present in leaves of the C4 species where is the predominant isoform. GgNAD-MEα/ß1 exhibits high catalytic efficiency and is differentially activated by the C4 intermediate aspartate. In C4 Cleome NAD-MEα/ß1 represents thus the C4-decarboxylase. GgNAD-MEß1and CaNAD-MEß1 are non-catalytic subunits but impart a stabilizing effect on the associated α-subunit. We conclude that in C4 Cleome the functions of NAD-ME as a TCA cycle associated enzyme and as a C4 photosynthetic decarboxylase coexist in BSC mitochondria and are performed by isoforms originated through associations of differentially adapted subunits. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-30 |
AnnouncementXML | Submission_2021-11-30_13:31:43.747.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD026547 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Gereon Poschmann |
SpeciesList | scientific name: Tarenaya hassleriana; NCBI TaxID: 28532; scientific name: Cleome angustifolia; NCBI TaxID: 860688; scientific name: Gynandropsis gynandra; NCBI TaxID: 190802; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-07 07:18:24 | ID requested | |
⏵ 1 | 2021-11-30 13:31:44 | announced | |
Publication List
H, ü, dig M, Tronconi MA, Zubimendi JP, Sage TL, Poschmann G, Bickel D, Gohlke H, Maurino VG, Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits. Plant Cell, 34(1):597-615(2022) [pubmed] |
Keyword List
submitter keyword: NAD-malic enzyme , C4-photosynthesis, respiration, plant, Cleomaceae, photosynthesis |
Contact List
Gereon Poschmann |
contact affiliation | Proteome Research (AG Stühler) Institute of Molecular Medicine I and Molecular Proteomics Laboratory BMFZ Heinrich Heine University Düsseldorf |
contact email | gereon.poschmann@hhu.de |
lab head | |
Gereon Poschmann |
contact affiliation | Molecular Proteomics Laboratory |
contact email | gereon.poschmann@hhu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026547
- Label: PRIDE project
- Name: Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cells mitochondria and evolved via association of differentially adapted subunits