PXD026499-1

PXD026499 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	LC-MS/MS-based in vitro kinase assays performed with recombinant maltose-binding protein-tagged P. falciparum calcium dependent protein kinase 4 using synthetic peptides as substrates
Description	In vitro kinase assays were performed with recombinant maltose-binding protein (MBP) tag-fused PfCDPK4 protein using synthetic peptides as substrates. Kinase reaction buffer contained CaCl2 or the calcium chelator EGTA. Triplicate reactions with either the CaCl2 buffer or the EGTA buffer were performed in parallel and the peptides were detected and quantified by LC-MS/MS. The suitability of a peptide as a substrate for PfCDPK4 was quantified as the depletion of the unmodified peptide as it was converted to phosphopeptide. Additionally, autophosphorylation of PfCDPK4 was assessed by performing the reaction in the absence of substrates followed by tryptic digest and LC-MS/MS of the resulting peptides to identify phosphorylation sites.
HostingRepository	MassIVE
AnnounceDate	2021-06-05
AnnouncementXML	Submission_2021-06-05_13:17:49.921.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristian Swearingen
SpeciesList	scientific name: Plasmodium falciparum; common name: malaria parasite P. falciparum; NCBI TaxID: 5833;
ModificationList	O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-05 12:23:14	ID requested
⏵ 1	2021-06-05 13:17:50	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: plasmodium falciparum, kinase, phosphorylation

submitter keyword: plasmodium falciparum, kinase, phosphorylation

Contact List

Kristian Swearingen
contact affiliation	Institute for Systems Biology
contact email	kristian.swearingen@systemsbiology.org
lab head
Kristian Swearingen
contact affiliation	Institute for Systems Biology
contact email	kristian.swearingen@isbscience.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087575/