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PXD026499-1

PXD026499 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS-based in vitro kinase assays performed with recombinant maltose-binding protein-tagged P. falciparum calcium dependent protein kinase 4 using synthetic peptides as substrates
DescriptionIn vitro kinase assays were performed with recombinant maltose-binding protein (MBP) tag-fused PfCDPK4 protein using synthetic peptides as substrates. Kinase reaction buffer contained CaCl2 or the calcium chelator EGTA. Triplicate reactions with either the CaCl2 buffer or the EGTA buffer were performed in parallel and the peptides were detected and quantified by LC-MS/MS. The suitability of a peptide as a substrate for PfCDPK4 was quantified as the depletion of the unmodified peptide as it was converted to phosphopeptide. Additionally, autophosphorylation of PfCDPK4 was assessed by performing the reaction in the absence of substrates followed by tryptic digest and LC-MS/MS of the resulting peptides to identify phosphorylation sites.
HostingRepositoryMassIVE
AnnounceDate2021-06-05
AnnouncementXMLSubmission_2021-06-05_13:17:49.921.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKristian Swearingen
SpeciesList scientific name: Plasmodium falciparum; common name: malaria parasite P. falciparum; NCBI TaxID: 5833;
ModificationListO-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; S-carboxamidomethyl-L-cysteine
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-05 12:23:14ID requested
12021-06-05 13:17:50announced
Publication List
no publication
Keyword List
submitter keyword: plasmodium falciparum, kinase, phosphorylation
Contact List
Kristian Swearingen
contact affiliationInstitute for Systems Biology
contact emailkristian.swearingen@systemsbiology.org
lab head
Kristian Swearingen
contact affiliationInstitute for Systems Biology
contact emailkristian.swearingen@isbscience.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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