PXD026499-1
PXD026499 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS-based in vitro kinase assays performed with recombinant maltose-binding protein-tagged P. falciparum calcium dependent protein kinase 4 using synthetic peptides as substrates |
Description | In vitro kinase assays were performed with recombinant maltose-binding protein (MBP) tag-fused PfCDPK4 protein using synthetic peptides as substrates. Kinase reaction buffer contained CaCl2 or the calcium chelator EGTA. Triplicate reactions with either the CaCl2 buffer or the EGTA buffer were performed in parallel and the peptides were detected and quantified by LC-MS/MS. The suitability of a peptide as a substrate for PfCDPK4 was quantified as the depletion of the unmodified peptide as it was converted to phosphopeptide. Additionally, autophosphorylation of PfCDPK4 was assessed by performing the reaction in the absence of substrates followed by tryptic digest and LC-MS/MS of the resulting peptides to identify phosphorylation sites. |
HostingRepository | MassIVE |
AnnounceDate | 2021-06-05 |
AnnouncementXML | Submission_2021-06-05_13:17:49.921.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kristian Swearingen |
SpeciesList | scientific name: Plasmodium falciparum; common name: malaria parasite P. falciparum; NCBI TaxID: 5833; |
ModificationList | O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; S-carboxamidomethyl-L-cysteine |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-06-05 12:23:14 | ID requested | |
⏵ 1 | 2021-06-05 13:17:50 | announced |
Publication List
no publication |
Keyword List
submitter keyword: plasmodium falciparum, kinase, phosphorylation |
Contact List
Kristian Swearingen | |
---|---|
contact affiliation | Institute for Systems Biology |
contact email | kristian.swearingen@systemsbiology.org |
lab head | |
Kristian Swearingen | |
contact affiliation | Institute for Systems Biology |
contact email | kristian.swearingen@isbscience.org |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087575/ |