PXD026464-1

PXD026464 is an original dataset announced via ProteomeXchange.

Title	Spatially restricted proteomics reveals dynamic changes in the caveolin-1 interactome in response to increased membrane tension
Description	Here, we used APEX2 proximity labelling technique coupled with mass spectrometry to define Cveolin-1 interactome at steady state and after hypo-osmotic shock. We created a stable cell line expressing Caveolin1-APEX2-EGFP in human retinal pigmented epithelial cells (RPE-1). Hypo osmotic shock was performed accordingly to Sinha et al, 2011. Briefly, cells were incubated in 30mOsm medium for 5 minutes (Hypo condition). To analyse the ability to recover, after the osmotic shock cells were allowed to recover in isotonic medium for 30 minutes (REC condition). Cells grown in iso-tonic medium were used as normal non treated condition (NT). RPE-1 cells expressing APEX2-EGFP in the cytoplasm (NES-APEX2-EGFP) was used as control to exclude highly abundant cytosolic proteins.
HostingRepository	PRIDE
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2024-11-05_07:01:25.746.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rossana Girardello
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-03 06:57:36	ID requested
⏵ 1	2024-11-05 07:01:29	announced

Martin E, Girardello R, Dittmar G, Ludwig A, Time-resolved proximity proteomics uncovers a membrane tension-sensitive caveolin-1 interactome at the rear of migrating cells. Elife, 13():(2024) [pubmed]

10.7554/elife.85601;

submitter keyword: Caveolin-1

APEX2

Interactome

Membrane Tension

Gunnar Dittmar
contact affiliation	Luxembourg Institute of Health 1A-B, rue Thomas Edison, L-1445 Strassen Luxembourg
contact email	gunnar.dittmar@lih.lu
lab head
Rossana Girardello
contact affiliation	Luxembourg Institute of Health
contact email	rossanagirardello@yahoo.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026464
     1. Label: PRIDE project
     2. Name: Spatially restricted proteomics reveals dynamic changes in the caveolin-1 interactome in response to increased membrane tension