PXD026450-2

PXD026450 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 spike protein glycosylation enriched by HILIC and Ti-IMAC
Description	In this work, we performed a fully descriptive analysis N- and O- linked glycosylation of SARS-COV-2 S glycoprotein. We investigated that dual-functionalized Ti-IMAC material enable the simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprotein from HEK293, which will eliminate the signal suppression of neutral glycopeptides to sialyl glycopeptides and improve the glycoform coverage of S protein. We have profiled 19 of its 22 potential N-glycosylated sites with 398 unique glycoforms in dual-functional Ti-IMIAC approach that is 1.6-fold of that in conventional HILIC method. We also identified O-linked glycosylation site that was not found in dual-functional Ti-IMIAC approach. In addition, we have also identified mannose-6-phosphate (M6P) glycosylation, which substantially expands the current knowledge of the spike protein’s glycosylation and enables the investigation of the influence of mannose-6-Phosphate on its cell entry.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:39.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junfeng Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-03 02:43:26	ID requested
1	2022-02-18 08:51:53	announced
⏵ 2	2023-11-14 08:52:40	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, Spike protein

Lingjun Li
contact affiliation	1School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA. Email: lingjun.li@wisc.edu 2Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53705, USA.
contact email	lingjun.li@wisc.edu
lab head
Junfeng Huang
contact affiliation	University of Wisconsin-Madison
contact email	jhuang373@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD026450

PRIDE project URI

• PRIDE
  1. PXD026450
     1. Label: PRIDE project
     2. Name: SARS-CoV-2 spike protein glycosylation enriched by HILIC and Ti-IMAC