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PXD026450-2

PXD026450 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSARS-CoV-2 spike protein glycosylation enriched by HILIC and Ti-IMAC
DescriptionIn this work, we performed a fully descriptive analysis N- and O- linked glycosylation of SARS-COV-2 S glycoprotein. We investigated that dual-functionalized Ti-IMAC material enable the simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprotein from HEK293, which will eliminate the signal suppression of neutral glycopeptides to sialyl glycopeptides and improve the glycoform coverage of S protein. We have profiled 19 of its 22 potential N-glycosylated sites with 398 unique glycoforms in dual-functional Ti-IMIAC approach that is 1.6-fold of that in conventional HILIC method. We also identified O-linked glycosylation site that was not found in dual-functional Ti-IMIAC approach. In addition, we have also identified mannose-6-phosphate (M6P) glycosylation, which substantially expands the current knowledge of the spike protein’s glycosylation and enables the investigation of the influence of mannose-6-Phosphate on its cell entry.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:52:39.475.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJunfeng Huang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-03 02:43:26ID requested
12022-02-18 08:51:53announced
22023-11-14 08:52:40announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19
submitter keyword: SARS-CoV-2, Spike protein
Contact List
Lingjun Li
contact affiliation1School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA. Email: lingjun.li@wisc.edu 2Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53705, USA.
contact emaillingjun.li@wisc.edu
lab head
Junfeng Huang
contact affiliationUniversity of Wisconsin-Madison
contact emailjhuang373@wisc.edu
dataset submitter
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Dataset FTP location
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