PXD026412-2

PXD026412 is an original dataset announced via ProteomeXchange.

Title	FIRST DETECTION OF SARS-COV-2 PROTEINS IN WASTEWATER SAMPLES BY MASS SPECTROMETRY
Description	On March 12, 2020, the World Health Organization (WHO) declared COVID-19 as a global pandemic. COVID-19 is produced by a novel β-coronavirus known as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) [1]. Several studies have detected SARS-CoV-2 RNA in urine, feces, and other biofluids from both symptomatic and asymptomatic people with COVID-19 [2], suggesting that SARS-CoV-2 RNA could be detected in human wastewater [3]. Thus, wastewater-based epidemiology (WBE) is now used as an approach to monitor COVID-19 prevalence in many different places around the world [4-10] . Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is the most common SARS-CoV-2 detection method in WBE, but there are other methods for viral biomolecule detection that could work as well. The aim of this study was to evaluate the presence of SARS-CoV-2 proteins in untreated wastewater (WW) influents collected from six wastewater treatment plants (WWTPs), from Durham Region, Ontario, Canada, using a LC-MS/MS-based proteomics approach. We identified many SARS-CoV-2 proteins in these wastewater samples, with peptides from pp1ab being the most consistently detected and with consistent abundance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:35:37.145.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Denina Simmons
SpeciesList	scientific name: Coronavirus PREDICT CoV-2; NCBI TaxID: 2042546;
ModificationList	No PTMs are included in the dataset
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-01 22:10:09	ID requested
1	2024-10-08 11:09:14	announced
⏵ 2	2024-10-22 05:35:37	announced	2024-10-22: Updated project metadata.

10.1021/ACS.EST.1C04705;

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, proteomics, liquid-chromatography mass spectrometry, wastewater, COVID-19

Denina Simmons
contact affiliation	Aquatic Omics Lab, Science department, Ontario Tech University
contact email	Denina.Simmons@ontariotechu.ca
lab head
Denina Simmons
contact affiliation	Ontario Tech University
contact email	denina.simmons@uoit.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD026412

PRIDE project URI

• PRIDE
  1. PXD026412
     1. Label: PRIDE project
     2. Name: FIRST DETECTION OF SARS-COV-2 PROTEINS IN WASTEWATER SAMPLES BY MASS SPECTROMETRY