PXD026356-1

PXD026356 is an original dataset announced via ProteomeXchange.

Title	Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay
Description	To search for the functional “m6A readers” specifically in the context of Drosophila early embryos, we incubated m6A-modified or unmodified RNA probes with cytosolic lysates from early embryos, and performed immunoprecipitation experiments followed by mass spectrometry analysis. We found that Drosophila FMRP (FMR1) was highly abundant in complexes immuno-precipitated by the m6A-modified probe. We performed data-independent acquisition (DIA) quantitative proteomics on the wild type and fmr1 maternal mutant embryos at multiple developmental stages, including 0-1 h, 2-3 h, and 5-6 h stages. To screen the FMR1-associated partners, we collected the 2–3-hour embryos and performed immunoprecipitation using the anti-Drosophila FMR1 antibody, followed by mass spectrometric analysis.
HostingRepository	iProX
AnnounceDate	2021-12-19
AnnouncementXML	Submission_2021-12-19_00:29:31.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoqiang Zhang
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-28 19:08:19	ID requested
⏵ 1	2021-12-19 00:29:32	announced

Dataset with its publication pending

submitter keyword: drosophila，embryo，m6A, fmr1

Dahua Chen
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	chendh@ioz.ac.cn
lab head
Guoqiang Zhang
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	gqzhang@ynu.edu.cn
dataset submitter

iProX dataset URI