PXD026352 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells |
Description | To gain insight into the function of DNA-PKcs within immune cells, we performed a quantitative phosphoproteomic screen in T cells to identify first order phosphorylation targets of DNA-PKcs. Results indicate that DNA-PKcs phosphorylates the transcription factor Egr1 (early growth response protein 1) at S301. Expression of Egr1 is induced early upon T cell activation and dictates T cell response by modulating expression of cytokines and key costimulatory molecules. Mutation of serine 301 to alanine via CRISPR-Cas9 resulted in increased proteasomal degradation of Egr1 and a decrease in Egr1-dependent transcription of IL2 (interleukin-2) in activated T cells. Our findings identify DNA-PKcs as a critical intermediary link between T cell activation and T cell fate and a novel phosphosite involved in regulating Egr1 activity. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-21 |
AnnouncementXML | Submission_2021-12-21_02:53:53.690.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aaron Storey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-28 09:00:12 | ID requested | |
⏵ 1 | 2021-12-21 02:53:54 | announced | |
2 | 2021-12-21 03:35:21 | announced | 2021-12-21: Updated publication reference for PubMed record(s): 34562454. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TMT, MS3, Phospo |
Contact List
Marie Burdine |
contact affiliation | Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR |
contact email | MBurdine@uams.edu |
lab head | |
Aaron Storey |
contact affiliation | University of Arkansas for Medical Sciences |
contact email | ajstorey@uams.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026352
- Label: PRIDE project
- Name: DNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells