PXD026343-1

PXD026343 is an original dataset announced via ProteomeXchange.

Title	Tensin3 interaction with talin drives formation of fibronectin-associated fibrillar adhesions
Description	The formation of healthy tissue involves continuous remodelling of the extracellular matrix (ECM). Whilst it is known that this requires integrin-associated cell-ECM adhesion sites (CMAs) and actomyosin-mediated forces, the underlying mechanisms remain unclear. Here we examine how tensin3 contributes to formation of fibrillar adhesions (FBs) and fibronectin fibrillogenesis. Using BioID mass spectrometry and a mitochondrial targeting assay, we establish that tensin3 associates with the mechanosensors talin and vinculin. We show that the talin R11 rod domain binds directly to a helical motif within the central intrinsically disordered region (IDR) of tensin3, whilst vinculin binds indirectly to tensin3 via talin. Using CRISPR knock-out cells in combination with defined tensin3 mutations, we show (i) that tensin3 is critical for formation of alpha5 beta1-integrin FBs and for fibronectin fibrillogenesis, and (ii) the talin/tensin3 interaction drives this process, with vinculin acting to potentiate it.
HostingRepository	PRIDE
AnnounceDate	2022-09-09
AnnouncementXML	Submission_2022-09-09_03:11:16.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suat Peng Neo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-28 02:48:38	ID requested
⏵ 1	2022-09-09 03:11:16	announced
2	2023-11-14 08:53:01	announced	2023-11-14: Updated project metadata.

10.1083/JCB.202107022;

submitter keyword: tensin, talin, vinculin, fibronectin, extracellular matrix

Jayantha Gunaratne
contact affiliation	Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, TBP Lab
contact email	jayanthag@imcb.a-star.edu.sg
lab head
Suat Peng Neo
contact affiliation	Institute of Molecular and Cell Biology (ASTAR)
contact email	neosp@imcb.a-star.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD026343

PRIDE project URI

• PRIDE
  1. PXD026343
     1. Label: PRIDE project
     2. Name: Tensin3 interaction with talin drives formation of fibronectin-associated fibrillar adhesions