PXD026311 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Alternative oxidase (AOX) seems to play a key role in embryogenic competence acquisition in Carica papaya L. calli |
Description | Understanding the mechanisms that endow a somatic cell with the ability to differentiate into a somatic embryo, which could result in numerous biotechnological applications, is still a challenge. The objective of this work was to identify some of the molecular and physiological mechanisms responsible for the acquisition of embryogenic competence during somatic embryogenesis in Carica papaya L. We performed a broad characterization of embryogenic (ECs) and nonembryogenic calli (NECs) of C. papaya using global and mitochondrial proteomic approaches, histomorphology, histochemistry, respiratory activity, and endogenous hormonal and hydrogen peroxide contents. ECs and NECs presented remarkable differences in anatomical and histochemical characteristics. ECs showed greater reactivity for the presence of proteins and neutral polysaccharides. Our results demonstrate the role of mitochondrial metabolism in the embryogenic competence of C. papaya calli. Greater participation of alternative oxidase (AOX) enzymes in respiration, as well as stronger accumulation of mitochondrial stress response proteins, was observed in ECs. In addition, ECs showed a greater abundance of proteins related to oxidative phosphorylation and higher total respiration (TR). Auxin-responsive Gretchen Hagen 3 (GH3) family proteins may play an important role in decreasing the contents of free 2,4-dichlorophenoxyacetic acid (2,4-D) in ECs. The accumulation of stress response proteins among total proteins was observed in ECs. ECs also showed higher endogenous hydrogen peroxide (H2O2) contents. H2O2 is a promising molecule for further investigation in differentiation protocols for C. papaya somatic embryos. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-28 |
AnnouncementXML | Submission_2022-06-27_20:20:02.455.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vanildo Silveira |
SpeciesList | scientific name: Carica papaya; NCBI TaxID: 3649; |
ModificationList | No PTMs are included in the dataset |
Instrument | SYNAPT G2-Si |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-27 08:03:40 | ID requested | |
⏵ 1 | 2022-06-27 20:20:03 | announced | |
Publication List
Vale EM, Santana DB, Reis RS, Sousa KR, de Souza Filho GA, Oliveira JG, Santa-Catarina C, Silveira V, Mitochondrial proteomics reveals new insights into embryogenic competence acquisition in Carica papaya L. callus. J Proteomics, 252():104434(2022) [pubmed] |
Keyword List
submitter keyword: Hormones, Label-free proteomics, Mitochondrial proteomics, Somatic embryos |
Contact List
Vanildo Silveira |
contact affiliation | Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil. |
contact email | vanildo@uenf.br |
lab head | |
Vanildo Silveira |
contact affiliation | UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE |
contact email | vanildo@uenf.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026311
- Label: PRIDE project
- Name: Alternative oxidase (AOX) seems to play a key role in embryogenic competence acquisition in Carica papaya L. calli