PXD026289-1

PXD026289 is an original dataset announced via ProteomeXchange.

Title	Sorting nexin-27 regulates AMPA receptor trafficking through the synaptic adhesion protein LRFN2
Description	The endosome-associated cargo adaptor sorting nexin-27 (SNX27) is linked to various neuropathologies through sorting of integral proteins to the synaptic surface, most notably AMPA receptors. To provide a broader view of SNX27-associated pathologies we performed proteomics in rat primary neurons to identify SNX27-dependent cargoes, and identified proteins linked to excitotoxicity, epilepsy, intellectual disabilities and working memory deficits. Focusing on the synaptic adhesion molecule LRFN2, we established that SNX27 binds to LRFN2 and regulates its endosomal sorting. Furthermore, LRFN2 associates with AMPA receptors and knockdown of LRFN2 results in decreased surface AMPA receptor expression, reduced synaptic activity, and attenuated hippocampal long-term potentiation. Overall, our study provides an additional mechanism by which SNX27 can control AMPA receptor-mediated synaptic transmission and plasticity indirectly through the sorting of LRFN2 and offers molecular insight into the perturbed function of SNX27 and LRFN2 in a range of neurological conditions.
HostingRepository	PRIDE
AnnounceDate	2021-06-28
AnnouncementXML	Submission_2021-06-28_06:08:58.291.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Boris Simonetti
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-26 07:35:34	ID requested
⏵ 1	2021-06-28 06:08:58	announced

Dataset with its publication pending

submitter keyword: SNX27, LRFN2, AMPA receptors, membrane trafficking

Pete Cullen
contact affiliation	School of Biochemistry Faculty of Life Sciences University of Bristol
contact email	pete.cullen@bristol.ac.uk
lab head
Boris Simonetti
contact affiliation	University of Bristol
contact email	bs13866@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD026289

PRIDE project URI

• PRIDE
  1. PXD026289
     1. Label: PRIDE project
     2. Name: Sorting nexin-27 regulates AMPA receptor trafficking through the synaptic adhesion protein LRFN2