PXD026288-1

PXD026288 is an original dataset announced via ProteomeXchange.

Title	Tumor temporal proteome profiling reveals the immunological triple offensive induced by synthetic anti-cancer Salmonella
Description	The engineered “obligate” anaerobic Salmonella typhimurium strain YB1 shows a prominent ability to repress tumor growth and metastasis, which has great potential as a novel cancer immunotherapy. However, the antitumor mechanism of YB1 remains unelucidated. To resolve the proteome dynamics induced by the engineered bacteria, we applied tumor temporal proteome profiling on murine bladder tumors after intravenous injection of either YB1 or PBS as a negative control. Our data suggests that during the two weeks treatment of YB1 injections, the cured tumors experienced three distinct phases of the immune response. Two days after injection, the innate immune response was activated, particularly the complement and blood coagulation pathways. In the meantime, the phagocytosis was initiated. The professional phagocytes such as macrophages and neutrophils were recruited, especially the infiltration of M1 macrophages was enhanced. Seven days after injection, substantial amount of T cells was observed at the invasion margin of the tumor. As a result, the tumor shrunk significantly. Overall, the temporal proteome profiling can systematically reveal the YB1 induced immune responses in tumor, showing great promise for elucidating the mechanism of bacteria-mediated cancer immunotherapy.
HostingRepository	PRIDE
AnnounceDate	2021-08-13
AnnouncementXML	Submission_2021-08-12_22:19:48.874.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhao wenjuan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-26 07:35:34	ID requested
⏵ 1	2021-08-12 22:19:49	announced

Dataset with its publication pending

submitter keyword: Quantitative proteomics, cancer immunotherapy, engineered Salmonella, blood coagulation, M1 macrophage, tumor specific T cell

Li Nan
contact affiliation	Dept. Chemical Proteomics, Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology (SIAT), Chinese Academy of Sciences
contact email	nan.li@siat.ac.cn
lab head
zhao wenjuan
contact affiliation	Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology (SIAT), Chinese Academy of Sciences
contact email	wj.zhao1@siat.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD026288

PRIDE project URI

• PRIDE
  1. PXD026288
     1. Label: PRIDE project
     2. Name: Tumor temporal proteome profiling reveals the immunological triple offensive induced by synthetic anti-cancer Salmonella