PXD026253-1

PXD026253 is an original dataset announced via ProteomeXchange.

Title	Analysis of the Putative Nucleoporin POM33 in the Filamentous Fungus Sordaria macrospora
Description	The POM33 protein of Sordaria macrospora is a nucleoporin considered of being an ER-protein that may function in ER-shaping and organization. In this study, we performed TagRFP-T pulldown experiments in S. macrospora strains expressing either POM33-TagRFP-T or the free TagRFP-T as control to identify putative POM33 interaction partners. Since with the SmSTRIPAK-component SCI1, POM33 was found, we expected with this approach to vice versa pull down SCI1. The results of the LC/MS analysis revealed mainly proteins that are located at the ER-membrane where they have functions in fatty acid biosynthesis, sterol biosynthesis, lipid metabolism or ER-shaping and maintaining its organization. These results gave us an overall picture about putative interactions of POM33 to specify its localization and function in S. macrospora hyphae.
HostingRepository	PRIDE
AnnounceDate	2021-08-23
AnnouncementXML	Submission_2021-08-23_06:53:46.925.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Sordaria macrospora; NCBI TaxID: 5147;
ModificationList	monohydroxylated residue; mono N-acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-25 06:57:17	ID requested
⏵ 1	2021-08-23 06:53:47	announced

Dataset with its publication pending

submitter keyword: STRIPAK,Sordaria macrospora. POM33, endoplasmatic reticulum

Stefanie Pöggeler
contact affiliation	Genetik eukaryotischer Mikroorganismen, Institut für Mikrobiologie und Genetik, Göttingen, Germany
contact email	spoegge@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026253
     1. Label: PRIDE project
     2. Name: Analysis of the Putative Nucleoporin POM33 in the Filamentous Fungus Sordaria macrospora