PXD026239-1

PXD026239 is an original dataset announced via ProteomeXchange.

Title	Proteins that co-purify with tandem affinity-purified PRC5 in Trypanosoma brucei
Description	The project was to determine the role of a trypanosomatid protein, termed PRC5, in pre-mRNA splicing. PRC5 has no homologs in model organisms but was recurrently co-purified with known splicing factors in Trypanosoma brucei. To determine interacting proteins, PRC5 was C-terminally TAP-tagged (we used the PTP tag) and tandem affinity-purified, and co-purifying proteins identified after separation by SDS-PAGE and in-gel trypsin digest by LC/MS/MS. Many of the enriched proteins were known splicing factors, and sucrose gradient sedimentation identified a complex of five subunits, termed PRP19-related complex or PRC.
HostingRepository	PRIDE
AnnounceDate	2021-12-15
AnnouncementXML	Submission_2021-12-15_07:44:20.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arthur Gunzl
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-24 21:57:11	ID requested
⏵ 1	2021-12-15 07:44:21	announced

Srivastava A, Ambr, ó, sio DL, Tasak M, Gosavi U, G, ü, nzl A, A distinct complex of PRP19-related and trypanosomatid-specific proteins is required for pre-mRNA splicing in trypanosomes. Nucleic Acids Res, 49(22):12929-12942(2021) [pubmed]

submitter keyword: Trypanosome, spliceosome, PRP19-related complex, Tandem affinity purification, LC/MS/MS

Arthur Gunzl
contact affiliation	Department of Genetics and Genome Sciences University of Connecticut Health Center 400 Farmington Avenue Farmington, CT 06030-6403 USA Phone (860) 679-8878 Fax (860) 679-8345 E-mail gunzl@uchc.edu
contact email	gunzl@uchc.edu
lab head
Arthur Gunzl
contact affiliation	UConn Health
contact email	gunzl@uchc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD026239

PRIDE project URI

• PRIDE
  1. PXD026239
     1. Label: PRIDE project
     2. Name: Proteins that co-purify with tandem affinity-purified PRC5 in Trypanosoma brucei