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PXD026229 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAxonal and somatodendritic proteomes of dopamine neurons in the mouse brain
DescriptionDopamine (DA) neurons modulate neural circuits and behaviors via dopamine release from expansive, long range axonal projections. The elaborate cytoarchitecture of DA neurons is embedded within complex brain tissues, making it difficult to access the DA neuronal proteome using conventional methods. Here, we demonstrate APEX2 proximity labeling within genetically targeted neurons in the mouse brain, enabling subcellular proteomics with cell type-specificity. By combining APEX2 biotinylation with mass spectrometry, we mapped the somatodendritic and axonal proteomes of DA neurons. Our dataset reveals the proteomic architecture underlying axonal transport, dopamine transmission, and axonal metabolism in DA neurons. We find a significant enrichment of proteins encoded by Parkinson’s disease-linked genes in dopaminergic axons, including proteins with previously undescribed axonal localization. Our proteomic datasets comprise a significant resource for axonal and DA neuronal cell biology, while the methodology developed here will enable future studies of other neural cell types.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeter Sims
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-24 10:21:17ID requested
12021-06-01 08:41:24announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, APEX2, dopamine neurons, axon
Contact List
Peter Sims
contact affiliationColumbia University Irving Medical Center
contact emailpas2182@columbia.edu
lab head
Peter Sims
contact affiliationColumbia University
contact emailpas2182@columbia.edu
dataset submitter
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Dataset FTP location
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