PXD026218-1

PXD026218 is an original dataset announced via ProteomeXchange.

Title	Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America
Description	Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains.
HostingRepository	PRIDE
AnnounceDate	2021-07-01
AnnouncementXML	Submission_2021-07-01_03:59:53.095.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Glauber Wagner
SpeciesList	scientific name: Clostridioides difficile str. A-027; NCBI TaxID: 1155748;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-24 03:47:14	ID requested
⏵ 1	2021-07-01 03:59:53	announced

de Melo Pac, í, fico D, Costa CL, Moura H, Barr JR, Maia GA, Filho VB, Moreira RS, Wagner G, Domingues RMCP, Quesada-G, ó, mez C, de Oliveira Ferreira E, de Castro Brito GA, Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities. Sci Rep, 11(1):13273(2021) [pubmed]

submitter keyword: Proteomics, Clostridioides difficile, rybotype, mass spectrometry, Latin America

Gerly Brito
contact affiliation	Department of Morphology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceara Brazil
contact email	gerlybrito@hotmail.com
lab head
Glauber Wagner
contact affiliation	Federal Univesity of Santa Catarina
contact email	glauber.wagner@ufsc.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026218
     1. Label: PRIDE project
     2. Name: Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America