PXD026217 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | OTS Development and E. coli Proteome LC-MSMS |
Description | Over the past twenty years, the development of orthogonal biological systems has sparked a revolution in our ability to study cellular physiology. Orthogonal translation systems (OTSs) enable site-specific incorporation of hundreds of non-standard amino acids, offering unprecedented access to the study of cellular mechanisms modulated by post-translational modifications (e.g. protein phosphorylation). Although development of phosphoserine-OTSs (pSerOTS) has been significant, little work has focused on the biology of OTS development and utilization. To better understand the impact of OTSs on host physiology, we utilize pSerOTS as a model to systematically explore the extent to which OTS components interact with Escherichia coli. Using this information, we constructed pSerOTS variants designed to enhance OTS orthogonality by minimizing interactions with host processes and decreasing stress response activation. Our expanded understanding of OTS:host interactions enables informed OTS design practices which minimize the negative impact of OTSs while improving OTS performance across a range of experimental settings. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-27 |
AnnouncementXML | Submission_2023-07-27_01:04:23.531.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | KyleMohler |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-24 03:47:08 | ID requested | |
⏵ 1 | 2023-07-27 01:04:24 | announced | |
2 | 2023-11-14 09:04:22 | announced | 2023-11-14: Updated project metadata. |
Publication List
Mohler K, Moen JM, Rogulina S, Rinehart J, System-wide optimization of an orthogonal translation system with enhanced biological tolerance. Mol Syst Biol, 19(8):e10591(2023) [pubmed] |
Keyword List
submitter keyword: LC-MSMS, OTS,Proteome, MS reporter |
Contact List
JesseRinehart |
contact affiliation | Department of Cellular & Molecular Physiology, Yale School of Medicine Systems Biology Institute, Yale University |
contact email | jesse.rinehart@yale.edu |
lab head | |
KyleMohler |
contact affiliation | Yale University |
contact email | kyle.mohler@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD026217 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026217
- Label: PRIDE project
- Name: OTS Development and E. coli Proteome LC-MSMS