PXD026208-2

PXD026208 is an original dataset announced via ProteomeXchange.

Title	SS18 regulates pluripotent-somatic transition through phase separation
Description	The transition from pluripotent to somatic states marks a critical event in mammalian development, but remains largely unresolved. Here we report the identification of SS18 as a regulator for pluripotent to somatic transition or PST by CRISPR-based whole genome screens. Mechanistically, SS18 forms microscopic condensates in nuclei through a C-terminal intrinsically disordered region (IDR) rich in tyrosine, which, once mutated, no longer form condensates nor rescue SS18-/- defect in PST. Yet, the IDR alone is not sufficient to rescue the defect even though it can form condensates indistinguishable from the wild type protein. We further show that its N-terminal 70aa is required for PST by interacting with BAF complex, and remains functional even swapped onto unrelated IDRs or even an artificial 24 tyrosine polypeptide. Finally, we show that SS18 mediates BAF assembly through phase separation to regulate PST. These studies suggest that SS18 plays a role in the pluripotent to somatic interface and undergoes liquid-liquid phase separation through a unique tyrosine-based mechanism.
HostingRepository	iProX
AnnounceDate	2021-05-23
AnnouncementXML	Submission_2023-02-06_19:24:27.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziwei Zhai
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-23 18:57:01	ID requested
1	2021-05-23 18:59:43	announced
⏵ 2	2023-02-06 19:24:28	announced	2023-02-07: Update publication information.

Kuang J, Zhai Z, Li P, Shi R, Guo W, Yao Y, Guo J, Zhao G, He J, Xu S, Wu C, Yu S, Zhou C, Wu L, Qin Y, Cai B, Li W, Wu Z, Li X, Chu S, Yang T, Wang B, Cao S, Li D, Zhang X, Chen J, Liu J, Pei D, SS18 regulates pluripotent-somatic transition through phase separation. Nat Commun, 12(1):4090(2021) [pubmed]

submitter keyword: SS18, BAF complex, phase separation, mouse embryonic stem cells, cell fate determination

Duanqing Pei
contact affiliation	Westlake University
contact email	peiduanqing@westlake.edu.cn
lab head
Ziwei Zhai
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	zwzhai@outlook.com
dataset submitter

iProX dataset URI