PXD026202-3

PXD026202 is an original dataset announced via ProteomeXchange.

Title	Chronic fluoxetine treatment of socially isolated rats modulates prefrontal cortex proteome
Description	Chronic social isolation (CSIS) is a risk factor for human major depressive disorder (MDD). Fluoxetine (Flx) is an antidepressant commonly used for first-line MDD therapy. However, its downstream mechanisms of action, beyond serotonergic signaling, remain elusive. We aimed to analyze the effects of CSIS followed by chronic Flx treatment on proteome changes in the adult male rat prefrontal cortex (PFC) cytosolic and non-synaptic mitochondria (NSM)-enriched fractions. Treatment with Flx ameliorated depression-like behaviors in CSIS-induced rat model of depression as assessed by sucrose preference test and forced swim test. Proteomic data showed that Flx increased the expression of proteins involved in cytoskeleton and regulation of intracellular Ca2+ homeostasis in the cytosol and of enzymes linking the glycolytic pathway to the citric acid cycle (Dlat) and ATPase-coupled ion transmembrane transport in NSM. CSIS resulted in down-regulation of cytosolic proteins involved in proteasome/ubiquitination processes and glutathione antioxidative system and up-regulated expression of key enzymes participating in oxidative phosphorylation. Presence of cytochrome c in the cytosol indicated compromised mitochondrial membrane integrity. Flx treatment in CSIS rats showed predominately an upregulation of vesicle-mediated transport proteins and reduced Uqcrfs1 along with transport in NSM, favoring reduced energy metabolism. Our data suggest that proteins from both cytosol and NSM-enriched fractions are affected by Flx in a key brain region associated with stress response, cognitive process and regulation of emotion.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:54.894.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026202
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dragana Filipovic
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-21 05:49:08	ID requested
1	2023-11-10 06:45:08	announced
2	2023-11-14 09:13:36	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:55	announced	2024-10-22: Updated project metadata.

10.1016/j.neuroscience.2022.08.011;

Filipovi, ć D, Novak B, Xiao J, Yan Y, Yeoh K, Turck CW, Chronic Fluoxetine Treatment of Socially Isolated Rats Modulates Prefrontal Cortex Proteome. Neuroscience, 501():52-71(2022) [pubmed]

10.6019/PXD026202;

submitter keyword: Depression,Fluoxetine, Prefrontal Cortex, Non-synaptic mitochondria, Energy metabolism

Dragana Filipovića
contact affiliation	Department of Molecular Biology and Endocrinology, “VINČA”, Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Serbia
contact email	dragana@vinca.rs
lab head
Dragana Filipovic
contact affiliation	Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Serbia
contact email	dragana@vinca.rs
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026202
     1. Label: PRIDE project
     2. Name: Chronic fluoxetine treatment of socially isolated rats modulates prefrontal cortex proteome