PXD026196-1

PXD026196 is an original dataset announced via ProteomeXchange.

Title	BioID-screening reveals the Alk “proximitome” of the Drosophila brain
Description	Anaplastic lymphoma kinase (Alk) is an evolutionary conserved receptor tyrosine kinase of the insulin receptor family. In addition to its well-studied role in cancer, numerous studies on invertebrate and vertebrate models reveal that Alk-signaling is associated with a variety of complex traits such as: regulation of growth and metabolism, hibernation, regulation of neurotransmitters, synaptic coupling, axon targeting, decision making, memory formation and learning, alcohol use disorder, as well as steroid hormone metabolism. To shed light on ALK-driven signaling processes, we used BioID-based in vivo proximity labeling to reveal the molecules that interact with Alk in the Drosophila CNS. Therefore, we generated first and next generation BioID fusion proteins in the endogenous Alk locus by CRISPR/Cas9 induced homology-directed repair (HDR) resulting in viable and fertile flies. Our results show (i) that the next generation of BioID proteins (TurboID and miniTurbo) outperform the first generation BirA* fusion in terms of labeling efficiency, and (ii) allow identification of proximitomes without overexpression which are likely to be more physiologically relevant. LC-MS/MS-based BioID screening of the larval brain revealed an extensive neuronal Alk-proximitome identifying numerous novel potential components of Alk signaling complexes. Validation of Alk-proximitome candidates further revealed co-expression with Alk in the CNS.
HostingRepository	PRIDE
AnnounceDate	2025-08-22
AnnouncementXML	Submission_2025-08-22_01:50:51.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Core Facility
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-21 05:48:34	ID requested
⏵ 1	2025-08-22 01:50:52	announced

U, ç, kun E, Wolfstetter G, Anthonydhason V, Sukumar SK, Umapathy G, Molander L, Fuchs J, Palmer RH, In vivo Profiling of the Alk Proximitome in the Developing Drosophila Brain. J Mol Biol, 433(23):167282(2021) [pubmed]

10.1016/j.jmb.2021.167282;

submitter keyword: TurboID,BirA*, CNS, MiniTurbo, signaling

Ruth Palmer
contact affiliation	Department of Medical Biochemistry and Cell Biology, Instititute of Biomedicine at the Sahlgrenska Academy, University of Gothenburg, SE-40530 Gothenburg, Sweden
contact email	Ruth.Palmer@gu.se
lab head
Proteomics Core Facility
contact affiliation	SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact email	gupcf@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD026196

PRIDE project URI

• PRIDE
  1. PXD026196
     1. Label: PRIDE project
     2. Name: BioID-screening reveals the Alk “proximitome” of the Drosophila brain