PXD026110-1

PXD026110 is an original dataset announced via ProteomeXchange.

Title	FASN-dependent de novo lipogenesis is required for brain development
Description	Fate and behaviour of neural progenitor cells is tightly regulated during mammalian brain development. Metabolic pathways, such as glycolysis and oxidative phosphorylation, that are required for supplying energy and providing molecular building blocks to generate cells, govern progenitor function. However, the role of de novo lipogenesis, which is the conversion of glucose into fatty acids through the multi-enzyme protein fatty acid synthase (FASN), for brain development remains unknown. Using Emx1Cre-mediated, tissue-specific deletion of Fasn in the mouse embryonic telencephalon, we show that loss of FASN causes severe microcephaly, largely due to altered polarity of apical, radial glia progenitors (APs) and reduced progenitor proliferation. Further, genetic deletion and pharmacological inhibition of FASN in human embryonic stem cell (ESC)-derived forebrain organoids identifies a conserved role of FASN-dependent lipogenesis for radial glia cell polarity and progenitor expansion in the developing human forebrain. Thus, our data establish a role of de novo lipogenesis for mouse and human brain development and identify a link between progenitor cell polarity and lipid metabolism.
HostingRepository	PRIDE
AnnounceDate	2022-01-14
AnnouncementXML	Submission_2022-01-14_13:24:50.698.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Gonzalez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-20 01:56:40	ID requested
⏵ 1	2022-01-14 13:24:51	announced
2	2023-11-14 08:53:01	announced	2023-11-14: Updated project metadata.

10.1073/PNAS.2112040119;

submitter keyword: Lipogenesis, FASN, brain development, brain organoids, mouse, human

Sebastian Jessberger
contact affiliation	University of Zurich, Brain Research Institute, Laboratory of Neural Plasticity
contact email	jessberger@hifo.uzh.ch
lab head
Daniel Gonzalez
contact affiliation	University of Zurich
contact email	gonzalez@hifo.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD026110

PRIDE project URI

• PRIDE
  1. PXD026110
     1. Label: PRIDE project
     2. Name: FASN-dependent de novo lipogenesis is required for brain development