PXD026060-1

PXD026060 is an original dataset announced via ProteomeXchange.

Title	Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly
Description	Cells respond to stress by blocking translation, rewiring metabolism, and forming transient mRNP assemblies called stress granules (SGs). After stress release, re-establishing homeostasis requires energy-consuming processes. However, the molecular mechanisms whereby cells restore energy production to disassemble SGs and reinitiate growth after stress remain poorly understood. Here we show that, upon stress, the ATP-producing enzyme Cdc19 forms inactive amyloids, and that their rapid re-solubilization is essential to restore energy production and SG disassembly. Cdc19 re-solubilization is initiated by the glycolytic metabolite fructose-1,6-bisphosphate (FBP), which directly binds Cdc19 amyloids and facilitates conformational changes that allow Hsp104 and Ssa2 chaperone recruitment. FBP then promotes Cdc19 tetramerization and activity, which together with trehalose breakdown boosts glycolysis to further enhance SG disassembly. Together, these results provide a molecular mechanism protecting cells during stress by directly coupling metabolism and ATP production with SG dynamics via regulation of Cdc19 amyloids.
HostingRepository	PRIDE
AnnounceDate	2021-08-17
AnnouncementXML	Submission_2021-08-17_02:02:46.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilaria Piazza
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-17 10:42:28	ID requested
⏵ 1	2021-08-17 02:02:46	announced

Dataset with its publication pending

submitter keyword: Cdc19, aggregation, SRM

Paola Picotti
contact affiliation	ETH Zurich
contact email	paola.picotti@imsb.biol.ethz.ch
lab head
Ilaria Piazza
contact affiliation	ETH Zurich
contact email	ilaria.piazza@bc.biol.ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026060
     1. Label: PRIDE project
     2. Name: Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly