PXD026009-2

PXD026009 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Facile one-pot nanoproteomics for label-free proteome profiling of 50-1000 mammalian cells
Description	We report a facile one-pot nanoproteomics method termed SOPs-MS (Surfactant-assisted One-Pot sample processing at the standard volume coupled with MS) for convenient proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOP-MS for label-free single-cell proteomics at low µL volume (Commun Bio 2021, 4, 265), we have systematically evaluated its processing volume at 10-200 µL using 100 human cells for robust reproducible nanoproteomic analysis. The processing volume of 50 µL which is in the range of volume for standard proteomics sample preparation, has been selected for easy sample handling with benchtop micropipette. Using the commonly accessible LC-MS platform, SOPs-MS allows for reliable label-free quantification of ~1,200-2,700 protein groups from 50-1000 MCF10A cells.
HostingRepository	jPOST
AnnounceDate	2021-07-23
AnnouncementXML	Submission_2022-09-18_02:42:21.830.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TaiTu Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxymethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-13 19:16:55	ID requested
1	2021-07-23 15:44:27	announced
⏵ 2	2022-09-18 02:42:22	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: SOPs-MS, nanoproteomics, small numbers of cells, surfactant DDM, colon crypt cells

submitter keyword: SOPs-MS, nanoproteomics, small numbers of cells, surfactant DDM, colon crypt cells

Contact List

Tujin Shi
lab head
TaiTu Lin
contact affiliation	PNNL
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001175/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001175/