PXD025986 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human PDR Vitreous Variability Study, TMT-MS |
Description | Control (n=27) and proliferative diabetic retinopathy (n=23) vitreous samples were treated as biologically distinct individuals or pooled together and aliquoted into technical replicates. Quantitative mass spectrometry with tandem mass tag labeling was used to identify proteins in individual or pooled control samples to determine technical and biological variability. To determine effect size and perform power analysis, control and proliferative diabetic retinopathy samples were analyzed across four 10plexes. Pooled samples were used to normalize the data across plexes and generate a single data matrix for downstream analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-14 |
AnnouncementXML | Submission_2022-02-14_02:29:05.380.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sarah Weber |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-12 08:56:01 | ID requested | |
⏵ 1 | 2022-02-14 02:29:06 | announced | |
Publication List
Weber SR, Zhao Y, Ma J, Gates C, da Veiga Leprevost F, Basrur V, Nesvizhskii AI, Gardner TW, Sundstrom JM, A validated analysis pipeline for mass spectrometry-based vitreous proteomics: new insights into proliferative diabetic retinopathy. Clin Proteomics, 18(1):28(2021) [pubmed] |
Keyword List
submitter keyword: human, vitreous, proliferative diabetic retinopathy, TMT-MS |
Contact List
Jeffrey M. Sundstrom |
contact affiliation | Department of Ophthalmology, Penn State College of Medicine |
contact email | jsundstrom@pennstatehealth.psu.edu |
lab head | |
Sarah Weber |
contact affiliation | Penn State College of Medicine |
contact email | srw5267@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025986 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025986
- Label: PRIDE project
- Name: Human PDR Vitreous Variability Study, TMT-MS