PXD025967-1

PXD025967 is an original dataset announced via ProteomeXchange.

Title	Deciphering the O-glycosylation of HKU1 Spike Protein with the Dual-Functional Hydrophilic Interaction Chromatography Materials
Description	HKU1 is a human betacoronavirus and infects host cells via highly glycosylated spike protein (S). At present, N-glycosylation of HKU1 S has been reported, however, little is known about its O-glycosylation, which hinders an in-depth understanding of its biological functions. Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica materials (HBS). The enrichment method for O-glycopeptides with HBS was developed and validated using standard proteins. The application of the developed method to HKU1 S1 subunit resulted in 61 novel O-glycosylation sites, among which 56% were predicted to be exposed on protein outer surface. Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were also analyzed. The obtained O-glycosylation dataset would provide valuable insights for the understanding of the structure of HKU1 S.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:17:39.354.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025967
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dongmei Fu
SpeciesList	scientific name: Human coronavirus HKU1 (isolate N1); NCBI TaxID: 443239;
ModificationList	complex glycosylation
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-12 01:45:48	ID requested
⏵ 1	2022-02-17 03:17:40	announced

Cui Y, Dong X, Zhang X, Chen C, Fu D, Li X, Liang X, Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials. Front Chem, 9():707235(2021) [pubmed]

submitter keyword: HKU1, spike glycoprotein, enrichment, O-glycosylation sites, O-glycosylation abundance

Dongmei Fu
contact affiliation	School of Bioengineering, Dalian Polytechnic University
contact email	dongmeifu@163.com
lab head
Dongmei Fu
contact affiliation	School of Bioengineering, Dalian Polytechnic University,
contact email	dongmeifu@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025967
     1. Label: PRIDE project
     2. Name: Deciphering the O-glycosylation of HKU1 Spike Protein with the Dual-Functional Hydrophilic Interaction Chromatography Materials