PXD025936-1

PXD025936 is an original dataset announced via ProteomeXchange.

Title	The termite fungal cultivar Termitomyces combines diverse enzymes and oxidative reactions for plant biomass conversion
Description	Macrotermitine termites have domesticated fungi in the genus Termitomycesas their primary food source using predigested plant biomass. To access the full nutritional value of lignin-enriched plant biomass, the termite-fungus symbiosis requires the depolymerization of this complex phenolic polymer. While most previous work suggests that lignocellulose degradation is accomplished predominantly by the fungal cultivar, our current understanding of the underlying biomolecular mechanisms remains rudimentary. Here, we provide conclusive omics and activity-based evidence that Termitomyces employs not only a broad array of carbohydrate-active enzymes (CAZymes) but also a restricted set of oxidizing enzymes (manganese peroxidase, dye decolorization peroxidase, an unspecific peroxygenase, laccases, and aryl-alcohol oxidases) and Fenton chemistry for biomass degradation. We propose for the first time that Termitomyces induces hydroquinone-mediated Fenton chemistry using a herein newly described 2-methoxy-1,4-dihy-droxybenzene (2-MH2Q, compound 19)-based electron shuttle system to complement the enzymatic degradation pathways. This study provides a comprehensive depiction of how efficient biomass degradation by means of this ancient insect’s agricultural symbiosis is accomplished.
HostingRepository	PRIDE
AnnounceDate	2021-05-12
AnnouncementXML	Submission_2021-05-11_22:25:05.554.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Termitomyces sp.; NCBI TaxID: 1916073;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-11 03:35:10	ID requested
⏵ 1	2021-05-11 22:25:05	announced

Dataset with its publication pending

submitter keyword: symbiosis, lignin degradation, Termitomyces, metabolites, redox chemistry, biodegradation, lignocellulose, redox proteins, secondary metabolism

Axel A. Brakhage
contact affiliation	Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Jena, Germany
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025936

PRIDE project URI

• PRIDE
  1. PXD025936
     1. Label: PRIDE project
     2. Name: The termite fungal cultivar Termitomyces combines diverse enzymes and oxidative reactions for plant biomass conversion