PXD025934-1

PXD025934 is an original dataset announced via ProteomeXchange.

Title	Detecting mistranslation in yeast expressing tRNA variants
Description	Mistranslation, the mis-incorporation of an amino acid not specified by the “standard” genetic code, occurs in all cells. tRNA variants that increase mistranslation arise spontaneously and engineered tRNAs can achieve mistranslation frequencies approaching 10% in yeast and bacteria. The goal of this study was to detect mistranslation from two different tRNA variants. The first variant, tRNA-Pro-G3:U70, has a mutation in its acceptor stem creating a G3:U70 base pair which is the key identity element for the alanine tRNA synthetase. This tRNA should be charged with alanine and mis-incorporate alanine at proline codons. The second variant, tRNA-Ser-UCU,G26A, is a serine tRNA with an arginine anticodon and a G26A secondary mutation to dampen function and prevent lethal levels of mistranslation. This tRNA should mis-incorporate serine at arginine codons.
HostingRepository	PRIDE
AnnounceDate	2021-05-17
AnnouncementXML	Submission_2021-05-16_22:58:35.957.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Berg
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-11 02:39:23	ID requested
⏵ 1	2021-05-16 22:58:36	announced

Dataset with its publication pending

submitter keyword: Mistranslation, tRNA variants, yeast, LC-MSMS

Dr. Judit Villen
contact affiliation	Department of Genome Science, University of Washington, USA
contact email	jvillen@uw.edu
lab head
Matthew Berg
contact affiliation	Department of Genome Sciences, University of Washington
contact email	mattberg@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025934
     1. Label: PRIDE project
     2. Name: Detecting mistranslation in yeast expressing tRNA variants