PXD025905-1

PXD025905 is an original dataset announced via ProteomeXchange.

Title	A comparative study of data-dependent acquisition and data-independent acquisition in proteomics analysis of clinical lung cancer tissues constrained by blood contamination
Description	Proteomics has been used extensively in life mechanism studies, drug target and clinical disease diagnosis, and treatment research.Several clinical surgery tissue samples inevitably get contaminated with blood during cryopreservation. This might detrimentally affect the results obtained by the commonly used proteomics DDA data acquisition strategy.For disease research, each clinical sample is precious and sample collection usually takes months or years. Removal of unqualified samples before the experiment is necessary but regrettable. The present findings demonstrate that for clinical tissue samples, compared with DDA strategy, the data derived using DIA is more complete and more potential biomarkers can be identified. DIA strategies can effectively overcome the interference from blood contaminants, allowing greater utilization of existing frozen samples. DIA has excellent analytical capability and application prospects.
HostingRepository	iProX
AnnounceDate	2021-05-10
AnnouncementXML	Submission_2021-05-09_21:19:06.204.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Su
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-09 21:16:39	ID requested
⏵ 1	2021-05-09 21:19:06	announced
2	2023-08-27 20:52:44	announced	2023-08-28: Update publication information.

Dataset with its publication pending

submitter keyword: proteomics, data-dependent acquisition (DDA),data-Independent acquisition (DIA),lung cancer, differentially expressed proteins

Meng Gong
contact affiliation	West China Hospital, Sichuan University
contact email	gongmeng@scu.edu.cn
lab head
Tao Su
contact affiliation	West China Hospital, Sichuan University
contact email	754436108@qq.com
dataset submitter

iProX dataset URI