PXD025893 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Elucidation of the Signatures of Proteasome-Catalyzed Peptide Splicing |
Description | Proteasomes catalyze the degradation of endogenous proteins into oligopeptides, but can concurrently create spliced oligopeptides through ligation of previously non-contiguous peptide fragments. Recent studies have uncovered a formerly unappreciated role for proteasome-catalyzed peptide splicing (PCPS) in the generation of non-genomically templated human leukocyte antigen class I (HLA-I)-bound cis-spliced peptides that can be targeted by CD8+ T cells in cancer and infection. However, the mechanisms defining PCPS reactions are poorly understood. Here, we experimentally define the biochemical constraints of proteasome-catalyzed cis-splicing reactions by examination of in vitro proteasomal digests of a panel of viral- and self-derived polypeptide substrates using a tailored mass-spectrometry-based de novo sequencing workflow. We show that forward and reverse PCPS reactions display unique splicing signatures, defined by preferential fusion of distinct amino acid residues with stringent peptide length distributions, suggesting sequence- and size-dependent accessibility of splice reactants for proteasomal substrate binding pockets. Our data provide the basis for a more informed mechanistic understanding of PCPS |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-17 |
AnnouncementXML | Submission_2021-05-17_11:50:51.592.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nicola Ternette |
SpeciesList | scientific name: Human immunodeficiency virus; NCBI TaxID: 12721; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-09 07:46:06 | ID requested | |
⏵ 1 | 2021-05-17 11:50:53 | announced | |
Publication List
Paes W, Leonov G, Partridge T, Nicastri A, Ternette N, Borrow P, Elucidation of the Signatures of Proteasome-Catalyzed Peptide Splicing. Front Immunol, 11():563800(2020) [pubmed] |
Keyword List
submitter keyword: Peptide spliicng, proteasome, antigen processing |
Contact List
Nicola Ternette |
contact affiliation | University of Oxford |
contact email | nicola.ternette@ndm.ox.ac.uk |
lab head | |
Nicola Ternette |
contact affiliation | University of Oxford |
contact email | nicola.ternette@ndm.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025893
- Label: PRIDE project
- Name: Elucidation of the Signatures of Proteasome-Catalyzed Peptide Splicing