PXD025874-1

PXD025874 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of healthy and hyperglycemic murine fetal kidneys
Description	Alteration of kidney morphogenesis in diabetic pregnancies is poorly described, especially changes in the extracellular matrix (ECM) and glomerular basement membrane (GBM during glomerulogenesis. Addressing the ECM proteome, or matrisome, in the mouse fetal kidney in a healthy and diabetic environment will improve understanding about the association between ECM changes in the kidney as potential reprogramming mechanisms of kidney differentiation in diabetic pregnancies. Therefore, to better understand ECM composition and remodelling in kidney developmental and appreciate the alterations associated to the maternal diabetes, this project aimed to define the matrisome in the mouse fetal kidney on embryonic day 19. For this, we used an ECM enrichment approach combined with high resolution label-free tandem mass spectrometry to define the matrisome in the fetal mouse kidney (healthy and hyperglycemic) to test the hypothesis that maternal diabetes influences ECM composition, assembly and, therefore, biology.
HostingRepository	PRIDE
AnnounceDate	2021-10-13
AnnouncementXML	Submission_2021-10-13_00:01:57.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louise Hopkinson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-07 04:26:22	ID requested
⏵ 1	2021-10-13 00:01:57	announced

Dataset with its publication pending

submitter keyword: Kidney, extracellular matrix, mass spectrometry, enrichment, basement membrane, diabetes

Rachel Lennon
contact affiliation	Wellcome Centre for Cell-matrix biology, University of Manchester, UK.
contact email	rachel.lennon@manchester.ac.uk
lab head
Louise Hopkinson
contact affiliation	University of Manchester
contact email	louise.hopkinson@postgrad.manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD025874

PRIDE project URI

• PRIDE
  1. PXD025874
     1. Label: PRIDE project
     2. Name: Proteomic analysis of healthy and hyperglycemic murine fetal kidneys