PXD025832-1

PXD025832 is an original dataset announced via ProteomeXchange.

Title	The interactome of site-specifically acetylated linker histone H1
Description	Linker histone H1 plays a key role in chromatin organization and maintenance, however, our knowledge of the regulation of H1 functions by its posttranslational modifications (PTMs) is very limited. In this study, we report on the generation of homogeneously and site-specifically mono- and di-acetylated H1 (H1 Ac) using genetic code expansion. We used these modified histones to identify and comprehensively characterize the acetylation-dependent cellular interactome for linker histone H1 and show that site-specific acetylation results in overlapping, but distinct groups of interacting partners. Intriguingly, H1 acetylation-specific interactors comprise translational initiation factors and are involved in transcriptional regulation, suggesting that acetylation of H1 may indeed act as a regulator of the linker histone H1 by modulation of protein-protein interactions.
HostingRepository	PRIDE
AnnounceDate	2021-07-09
AnnouncementXML	Submission_2021-07-08_21:37:40.955.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	micrOTOF II; Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 02:20:34	ID requested
⏵ 1	2021-07-08 21:37:41	announced

Dataset with its publication pending

submitter keyword: Linker histone H1, acetylation, posttranslational modification (PTM), histone code, epigenetics, chromatin, genetic code expansion, affinity purification-mass spectrometry (AP-MS), interactions

Florian Stengel
contact affiliation	Department of Biology – Cellular Proteostasis and Mass Spectrometry, University of Konstanz
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025832

PRIDE project URI

• PRIDE
  1. PXD025832
     1. Label: PRIDE project
     2. Name: The interactome of site-specifically acetylated linker histone H1