PXD025829 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Proteome-Wide Atlas of Lysine-Reactive Chemistry |
Description | Recent advances in chemical proteomics have begun to characterize the reactivity and ligandability of lysines on a global scale. Yet, only a limited diversity of aminophilic electrophiles have been evaluated for interactions with the lysine proteome. Here, we report an in-depth profiling of >30 uncharted aminophilic chemotypes that greatly expands the content of ligandable lysines in human proteins. Aminophilic electrophiles showed disparate proteomic reactivities that range from selective interactions with a handful of lysines to, for a set of dicarboxaldehyde fragments, remarkably broad engagement of the covalent small molecule-lysine interactions captured by the entire library. We used these latter “scout� electrophiles to efficiently map ligandable lysines in primary human immune cells under stimulatory conditions. Finally, we show that aminophilic compounds perturb diverse biochemical functions through site-selective modification of lysines in proteins, including protein-RNA interactions implicated in innate immune responses. These findings support the broad potential of covalent chemistry for targeting functional lysines in the human proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-21 |
AnnouncementXML | Submission_2021-09-21_02:02:54.630.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mikail Abbasov |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-06 01:34:41 | ID requested | |
1 | 2021-09-20 10:12:07 | announced | |
⏵ 2 | 2021-09-21 02:02:55 | announced | 2021-09-21: Updated project metadata. |
Publication List
Abbasov ME, Kavanagh ME, Ichu TA, Lazear MR, Tao Y, Crowley VM, Am Ende CW, Hacker SM, Ho J, Dix MM, Suciu R, Hayward MM, Kiessling LL, Cravatt BF, A proteome-wide atlas of lysine-reactive chemistry. Nat Chem, 13(11):1081-1092(2021) [pubmed] |
10.1038/S41557-021-00765-4; |
Keyword List
submitter keyword: Human immune cells, lysine reactive probes |
Contact List
Benjamin F. Cravatt |
contact affiliation | Department of Chemistry, Scripps Research Institute |
contact email | cravatt@scripps.edu |
lab head | |
Mikail Abbasov |
contact affiliation | Department of Chemistry and Chemical Biology Cornell University |
contact email | mikail.abbasov@cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD025829 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025829
- Label: PRIDE project
- Name: A Proteome-Wide Atlas of Lysine-Reactive Chemistry