PXD025818 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human cytomegalovirus-induced host protein citrullination is crucial for viral replication |
Description | Citrullination is the conversion of arginine-to-citrulline by protein arginine deiminases (PADs), whose dysregulation is implicated in the pathogenesis of various types of cancers and autoimmune diseases. Consistent with the ability of human cytomegalovirus (HCMV) to induce post-translational modifications of cellular proteins to gain a survival advantage, we show that HCMV infection of primary human fibroblasts triggers PAD-mediated citrullination of several host proteins, and that this activity promotes viral fitness. Citrullinome analysis reveals significant changes in deimination levels of both cellular and viral proteins, with interferon (IFN)-inducible protein IFIT1 being the most heavily deiminated one. As genetic depletion of IFIT1 strongly enhances HCMV growth, and in vitro IFIT1 citrullination impairs its ability to bind to 5’-pppRNA, we propose that viral-induced IFIT1 citrullination is a novel mechanism of HCMV evasion from host antiviral resistance. Overall, our findings point to a crucial role of citrullination in subverting cellular responses to viral infection. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-20 |
AnnouncementXML | Submission_2021-07-20_02:54:12.840.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eranthie Weerapana |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-05 22:41:31 | ID requested | |
⏵ 1 | 2021-07-20 02:54:13 | announced | |
Publication List
Griffante G, Gugliesi F, Pasquero S, Dell'Oste V, Biolatti M, Salinger AJ, Mondal S, Thompson PR, Weerapana E, Lebbink RJ, Soppe JA, Stamminger T, Girault V, Pichlmair A, Oroszl, á, n G, Coen DM, De Andrea M, Landolfo S, Human cytomegalovirus-induced host protein citrullination is crucial for viral replication. Nat Commun, 12(1):3910(2021) [pubmed] |
Keyword List
submitter keyword: citrullination |
Contact List
Eranthie Weerapana |
contact affiliation | Boston College |
contact email | eranthie@bc.edu |
lab head | |
Eranthie Weerapana |
contact affiliation | Boston College |
contact email | eranthie@bc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025818 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025818
- Label: PRIDE project
- Name: Human cytomegalovirus-induced host protein citrullination is crucial for viral replication