PXD025759-1

PXD025759 is an original dataset announced via ProteomeXchange.

Title	A novel method for creating a synthetic L-DOPA proteome and in vitro evidence of incorporation.
Description	Proteinopathies are protein misfolding diseases that have an underlying factor that effect the conformation of proteoforms. A factor hypothesised to play a role in these diseases is the incorporation of non-protein amino acids into proteins, with a key example being the therapeutic drug levodopa. The presence of levodopa as a protein constituent has been explored in several studies but it has not been examined in a global proteomic manner. This paper provides a proof-of-concept method for enzymatically creating levodopa containing proteins using the enzyme tyrosinase, provides spectral evidence of in vitro incorporation and the induction of the unfolded protein response due to levodopa.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:39:52.925.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025759
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joel Steele
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; L-3',4'-dihydroxyphenylalanine; hydroxylated residue; monohydroxylated residue; deaminated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-03 00:01:31	ID requested
⏵ 1	2024-10-07 13:39:53	announced

10.6019/PXD025759;

10.3390/proteomes9020024;

Steele JR, Strange N, Rodgers KJ, Padula MP, A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation. Proteomes, 9(2):(2021) [pubmed]

submitter keyword: Misincorporation

post translational modifications

PTM

Levodopa

L-DOPA

Kenneth J Rodgers
contact affiliation	Neurotoxin Research Group, School of Life Sciences, The University of Technology Sydney, Ultimo, NSW 2007, Australia; kenneth.rodgers@uts.edu.au (K.J.R.)
contact email	Kenneth.Rodgers@uts.edu.au
lab head
Joel Steele
contact affiliation	University of Technology Sydney
contact email	joel.r.steele@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025759

PRIDE project URI

• PRIDE
  1. PXD025759
     1. Label: PRIDE project
     2. Name: A novel method for creating a synthetic L-DOPA proteome and in vitro evidence of incorporation.